Fig. 6.

Effects of adenovirus-mediated transforming growth factor beta-stimulated clone 22 (TSC-22) gene delivery in normal rat hearts. TSC-22 a mRNA levels measured by RT-PCR 3 days or 2 weeks (n = 5–8) and b total protein levels analysed by Western blot (n = 8) 3 days after gene delivery in left ventricular (LV) tissue samples of Sprague–Dawley (SD)-rat hearts. c Localization of TSC-22 at cellular level was studied by immunohistochemical staining against TSC-22 at day 3 after gene transfer. d mRNA levels of B-type natriuretic peptide (BNP), atrial natriuretic peptide (ANP), interleukin-6 (IL-6), connective tissue growth factor (CTGF), collagen type Iα1 (col1a1), fibronectin-1 (Fn-1), plasminogen activator inhibitor-1 (PAI-1), Bcl-2-associated X protein (Bax2), B-cell lymphoma 2A (Bcl2A) and collagen type IIIα1(col3a1) measured by RT-PCR from LV of SD-rat hearts 2 weeks after gene delivery (n = 5). *P <0.05, **P <0.01, ***P <0.001 versus LacZ (Student’s t-test). The results are expressed as mean ± SEM. Representative Western blots are shown