Table 1.
Sequences of primers and fluorogenic probes used in real time quantitative RT-qPCR analysis (sequences 5′ to 3′)
| Gene | Forward Primer | Reverse Primer | Fluorogenic Probe |
|---|---|---|---|
| TSC-22 | GCAGGAGAACAATC | TGCAGCTGGG | CCAGTCCGGAG |
| TGTTGAAGAC | CCTGAAAC | CAGCTCGCC | |
| ANP | GAAAAGCAAACTG | CCTACCCCCG | TCGCTGGCCCT |
| AGGGCTCTG | AAGCAGCT | CGGAGCCT | |
| BNP | TGGGCAGAAGATAG | ACAACCTCAG | CGGCGCAGTCA |
| ACCGGA | CCCGTCACAG | GTCGCTTGG | |
| IL-6 | ATATGTTCTCAGGG | TGCATCATCGC | CAGAATTGCCATTGCA |
| AGATCTTGGAA | TGTTCATACAA | CAACTCTTTTCTCA | |
| CTGF | CGCCAACCGCAAGA | CACGGACCCA | CACTGCCAAAGA |
| TTG | CCGAAGAC | TGGTGCACCCTG | |
| PAI-1 | GCTGACCACAGCAG | GTGCCCCTCTCA | CCCGGCAGCAGAT |
| GGAAA | CTGATATTGAA | CCAAGATGCTAT | |
| col1a1 | CCCCTTGGTCTTGG | GCACGGAAAC | CTTTGCTTCCCAGATGT |
| AGGAA | TCCAGCTGAT | CCTATGGCTATGATG | |
| Fn-1 | GCGAGGCAGGATC | CCAATCTTGTA | ACCATTGCAAAT |
| AGCTG | GGACTGACCCC | CGCTGCCATGAA | |
| Bax2 | TCCCCCCGAGAGG | CCCAGTTGAAG | CCGGGTGGCAGCT |
| TCTTCT | TTGCCATCA | GACATGTTTG | |
| Bcl2A | GGAACTATATGG | AGGCTGAGCA | CGACCTCTGTTTG |
| CCCCAGCAT | GGGTCTTCAG | ATTTCTCCTGGCTGTC | |
| col3a1 | AGCTGGCCTTCCTC | GCTGTTTTTGCAG | TTCCAGCCGG |
| AGACTTC | TGGTATGTAATG | GCCTCCCAG | |
| 18S | TGGTTGCAAAGCT | AGTCAAATTA | CCTGGTGGTGC |
| GAAACTTAAAG | AGCCGCAGGC | CCTTCCGTCA |
TSC-22 transforming growth factor beta-stimulated clone 22; ANP atrial natriuretic peptide; BNP B-type natriuretic peptide; IL-6 interleukin-6; CTGF connective tissue growth factor; PAI-1 plasminogen activator inhibitor-1; col1a1 collagen type Iα1; Fn-1 fibronectin-1; Bax2 Bcl-2-associated X protein; Bcl2A B-cell lymphoma 2A; col3a1 collagen type IIIα1