Table 2.
Effect of intramyocardial TSC-22 gene delivery on cardiac function in the normal adult rat heart
| Variable | Group | TSC-22 | TSC-22 |
|---|---|---|---|
| 3 days | 2 weeks | ||
| Interventricular septum diastole (mm) | LacZ | 1.9 ± 0.1 | 2.0 ± 0.1 |
| TSC-22 | 2.1 ± 0.1 | 2.0 ± 0.1 | |
| Interventricular septum systole (mm) | LacZ | 2.6 ± 0.1 | 2.5 ± 0.2 |
| TSC-22 | 2.7 ± 0.2 | 2.8 ± 0.2 | |
| Left ventricle diastole (mm) | LacZ | 7.6 ± 0.3 | 8.6 ± 0.1 |
| TSC-22 | 7.2 ± 0.3 | 8.7 ± 0.2 | |
| Left ventricle systole (mm) | LacZ | 5.1 ± 0.3 | 6.1 ± 0.3 |
| TSC-22 | 4.7 ± 0.3 | 6.1 ± 0.3 | |
| Posterior wall distole (mm) | LacZ | 2.3 ± 0.2 | 1.6 ± 0.1 |
| TSC-22 | 2.0 ± 0.3 | 1.5 ± 0.1 | |
| Posterior wall systole (mm) | LacZ | 2.1 ± 0.2 | 2.3 ± 0.2 |
| TSC-22 | 2.1 ± 0.1 | 2.3 ± 0.1 | |
| Ejection fraction (%) | LacZ | 66.7 ± 2.3 | 58.2 ± 4.5 |
| TSC-22 | 69.1 ± 3.0 | 62.8 ± 3.0 | |
| Fractional shortening (%) | LacZ | 32.8 ± 1.7 | 27.4 ± 2.7 |
| TSC-22 | 34.7 ± 2.5 | 30.3 ± 2.0 | |
| IVRT | LacZ | no data | 24.8 ± 2.2 |
| TSC-22 | no data | 25.0 ± 1.6 | |
| E/A-ratio | LacZ | no data | 5.1 ± 0.8 |
| TSC-22 | no data | 2.7 ± 0.6* | |
| n = 7–8 | n = 5 |
Adenoviral gene construct expressing TSC-22 or LacZ were injected into LV free wall and echocardiographic measurements were performed at 3 days and 2 weeks after gene transfer. The results are expressed as mean ± SEM. *P < 0.05 versus LacZ (Student’s t-test). IVRT, isovolumic relaxation time