Fig. 5.

Weekly intravitreal injections and daily eye drop application of PEDF-34 promote RGC axon regeneration at 21 days after ONC. Representative GAP-43⁺ immunohistochemistry to show axons growing in the optic nerve after (A), ONC + PBS, (B), ONC + weekly intravitreal injections of PEDF-34 and (C), ONC + daily eye drops of PEDF-34 (scale bars = 100 μm). (D) Quantification of the number of GAP-43⁺ axons growing in the distal optic nerve stump at 250, 500, 1000, 1500, 2000 and 2500 μm from the lesion centre. * = P < 0.05, *** = P < 0.0001, ivit = intravitreal. Red arrow = lesion epicentre.