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. 2015 Oct 10;43(18):e117. doi: 10.1093/nar/gkv563

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — (A) The relative locations of M.TaqI labelling sites along λ-DNA. In total there are 121 sites on the 48.5 kbp long λ-DNA genome, each represented by a green mark. The length scale is given in bp below the cartoon. Above the molecule there is a ‘zoom-in’ representation of the labelling enzyme recognition site: TCGA and the fluorophore attached to the adenine base. (B) A simulated image showing the fluorescence intensity modulation along the image of a labelled λ-DNA genome (assuming that a point spread function spans 1500 bp). The total observed fluorescence is the convolution of overlapping point spread functions of neighbouring fluorophores. (C) A theoretical profile representing the expected amplitude modulations (AM) of a labelled λ-DNA genome under the same conditions as in (B).