Figure 4.

Conversion yields from linear gpPCR products to covalently closed dumbbell DNA. gpPCR products were either treated with ligase and exonuclease or not, and subjected to 10% PAGE. Band intensities of the ethidium bromide stained gels were quantified using ImageJ 1.37v software (NIH, USA). (A) hp-primer PCR products. (B) AP1-loop-primer PCR product. (C) Anti-GFP-shRNA expressing dumbbell db-Nick produced with the nicking-enzyme method. (D) Anti-luciferase-shRNA expressing dumbbell db-ELAN produced with the ELAN method.