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Purity of dumbbell vectors produced using different technologies. Db-AP1-hp and db-AP3-hp: gpPCR-produced dumbbells using primers AP1-hp or AP3-hp; db-ELAN: ELAN-produced dumbbell; db-Nick: egfp-targeting dumbbell produced using the nicking-enzyme method. (A) 10% PAGE analysis. (B) High-resolution capillary gel electrophoresis. The indicated purity in% refers to the total fraction of dumbbell vector DNA in the analyzed sample.
