Table 1. Calculation of costs, excluding those for column purification, for the production of 1 μg dumbbell DNA based on local prices in Singapore Dollar (SGD) converted into US Dollar (USD).
| ELAN db | Nicking-enzyme db | AP1-hp db | AP1-hp (exo-) db | AP3-hp db | AP1-loop db | |
|---|---|---|---|---|---|---|
| Oligodeoxyribonucleotides (pmol) | FwP: 330 | 1st round PCR | 5′P-FwP: 65 | 5′P-FwP: 65 | 5′P-FwP: 94 | 5′P-FwP: 94 |
| RvP: 110 | FwP: 5.6 | 5′P-RvP: 22 | 5′P-RvP: 22 | 5′P-RvP: 31 | 5′P-RvP: 31 | |
| Loop1: 70 | RvP: 5.6 | |||||
| Loop2: 70 | 2nd round PCR | |||||
| FwP: 70 | ||||||
| RvP: 70 | ||||||
| DNA polymerase (units) | Pfu: 11 | 1st round PCR | Pfu: 2.2 | Pfu: 2.2 | Pfu: 3.1 | Pfu: 3.1 |
| Taq: 22 | Taq: 0.7 | Taq: 4.4 | Taq: 4.4 | Taq: 6.3 | Taq: 6.3 | |
| 2nd round PCR | ||||||
| Taq: 8.8 | ||||||
| Restriction endonuclease (units) | XhoI: 9.7 | NB. Bpu 10I: 8.8 | ||||
| EcoRI: 9.7 | ||||||
| SalI: 4.2 | ||||||
| MfeI: 4.2 | ||||||
| DNA ligase (units) | T4 DNA ligase: 28 | T4 DNA ligase: 17.5 | T4 DNA ligase: 10.9 | T4 DNA ligase: 10.9 | T4 DNA ligase: 31.3 | CircLigase: 555 |
| T7 DNA polymerase (units) | 28 | 17.5 | 10.9 | 15.6 | 13.3 | |
| Total expensesa (USD) | 72.5 | 21.1 | 13.2 | 7.0 | 23.8 | 211.1 |
aBased on an exchange rate of 1 USD = 1.25 SGD.