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. 2015 Oct 10;43(18):8964–8972. doi: 10.1093/nar/gkv609

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — rlmCD (SP_1029) is responsible for m⁵U747 formation. (A) LC/MS analysis of RNaseT1 digest of rRNAs prepared from strains TIGR4 (WT; top row of the panels), Sp332 (ΔRlmCD; middle row) and Sp361 (ΔRlmCD/pRlmCD; bottom row). Left and right panels show mass chromatographic detection of singly-charged ions of 3-mer fragment m⁵U747 (m/z 984.12) and U747 (m/z 970.10), respectively. (B) Collision-induced dissociation (CID) spectra of RNA fragments bearing m⁵U747 (left panel) and U747 (right panel). The singly-charged ions of the 3-mer fragment carrying m⁵U747 (m/z 984.12) and U747 (m/z 970.11) were used as parent ions for CID.