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. 2015 Oct 10;43(18):8964–8972. doi: 10.1093/nar/gkv609

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Effect of RlmCD-depletion on RlmA^II. Whole cell lysates were prepared from strains TIGR4 (WT), Sp303 (ΔRlmA^II) and Sp332 (ΔRlmCD) (A) and Sp322 (ΔRlmA^II/pRlmA^II) and Sp370 (ΔRlmCD/pRlmA^II) (B) grown in BHIY broth to A₆₀₀ of 1.0 at 37°C and 5% CO₂ in air, and separated on SDS-11% polyacrylamide gels, with RlmA^II (5 ng) being the positive control. Proteins were immunostained with RlmA^II antibody.