Skip to main content
. 2015 Oct 10;43(18):8790–8800. doi: 10.1093/nar/gkv764

Figure 1.

Figure 1.

The N-terminal domain of RPA1 interacts with WRN and DNA2. (A) A gel showing the GST fusions of various parts of Xenopus RPA. The proteins were separated on a 12% SDS-PAGE gel and stained by Coomassie brilliant blue. The size markers are in kilodaltons. (B) A Western blot showing the interaction between Xenopus WRN and RPA1N. The various GST fusion proteins were incubated with the N-terminal 455 amino acids of WRN and then isolated by glutathione Sepharose beads. The proteins bound to beads were analyzed for WRN by Western blot. (C) A western blot showing the interaction between RPA1N (GST-1N) with WRN and DNA2 in Xenopus egg extracts. (D). A western blot showing the interaction between RPA1N (GST-1N) and DNA2.