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The location of RPA1N is important for the resection of ds-DNA. The substrate for resection was a 5.7 kb linearized ds-DNA with a ddC at the 3′ end and a 32P label immediately inside. The substrate was incubated with mock depleted or RPA depleted extracts supplemented with various RPA proteins or buffer. Samples taken at the indicated times were analyzed by a 1% TAE-agarose gel electrophoresis.
