Figure 6.

Nucleosome position at DAFC-66D is largely determined by primary DNA sequence. (A) Comparison of observed nucleosome occupancy at DAFC-66D in follicle cells in stage 10 (dark blue) with predicted nucleosome occupancy (light blue) based on nucleosome DNA sequence preferences (104,105). Red asterisks indicate three predicted nucleosome positions that were less occupied in vivo than predicted. (B and C) Expanded view of predicted and observed nucleosome occupancy at ACE3 (B) and Ori-β (C), with nucleotide composition plotted above (see color key). Nucleosome occupied sites are relatively GC rich (black and blue), while nucleosome depleted regions in ACE3 and Ori-β contain extended poly A:T tracts (red and green) that correspond to ORC binding sites.