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. 2015 Oct 10;43(18):8762–8773. doi: 10.1093/nar/gkv775

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Telomeric transcripts are localized around centrosome in syncytial embryos. Confocal images of 0−2-h-old embryos with the indicated genotypes. RNA FISH of HeT-A antisense probe revealed HeT-A RNA (green) near centrosomes in the prophase of twinKD^nos embryos (A), during metaphase-anaphase in twinKD^nos, Trf2/Trf2, rhiKD^nos and spnEKD^nos embryos (B) and in the cortex of wocKD^nos and twinKD^nos embryos (C). Red: γ-tubulin; blue: DNA. Bar: 10 μm. (D) The scheme depicts mitotic defects in early developmental stages as a result of HeT-A derepression in the germline and the accumulation of abundant HeT-A transcripts around centrosomes in embryos.