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. 2015 Oct 10;43(18):8830–8838. doi: 10.1093/nar/gkv799

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Phenotypes of the ctf18–2A allele. (A) Serial dilutions of control cells (W303–1a), rad53Δ (YAC53), ctf18Δ (YVM164), CTF18::K.l.TRP1 (wild type CTF18 but with the K.l.TRP1 marker inserted into the genomic locus, after the STOP codon of CTF18; YLG316) and ctf18–2A (also with the K.l.TRP1 marker inserted after the STOP codon of CTF18; YLG320) were plated on YPD medium, or YPD supplemented with 150mM hydroxyurea (HU), then grown for 2 days at 30°C. (B) Control cells (CTF18-K.l.TRP1; YLG445), ctf18–2A (ctf18–2A K.l.TRP1; YLG447) and ctf18Δ::K.l.TRP1 (YLG449), all expressing the Tet-repressor fused to GFP and with an array of the Tet-operator sites at the ura3 locus, were grown at 30°C and then arrested in G2-M phase by addition of nocodazole. Defects in sister chromatid cohesion were scored microscopically, by examining 100 cells and determining the percentage with two dots of TetR-GFP instead of one (examples of each class are shown in the upper panels). (C) Diploid cells with the genotype ctf18–2A / CTF18 mrc1Δ / MRC1 (YLG292) were sporulated and then subjected to tetrad analysis on YPD medium. The image was taken after two days growth at 30°C. (D) Tetrad analysis of the meiotic progeny of ctf18–2A / CTF18 ctf4Δ / CTF4 (YLG263).