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. 2015 Oct 10;43(18):8973–8989. doi: 10.1093/nar/gkv809

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Identification and Y2H characterization of yeast Prp31p mutants. (A) Schematic representation of the insertional mutagenesis screen. Random insertion of MuA and excision by NotI digestion and religation leave a 5 nucleotide insertion at site of integration. (B) Schematic representation of Prp31p domains and location of the insertions that were identified in primary two-hybrid screen. (C) Summary of the two-hybrid validation screen. ‘+’ and ‘-’ indicate the presence or absence of interactions. The numbers of the pACTII plasmids indicate the amino-acid after which the 5 nucleotide insertion occurred in yPRP31. The proteins encoded by pAS2 plasmids are indicated, with in parenthesis the name of the human homologs. (D) Two-hybrid interactions in WT yeast strain or in yeast strains deleted for Rsa1 (ΔRSA1) or Pih1 (ΔPIH1). The pACTII-yPRP31 plasmid was introduced in the indicated strain and tested against the indicated proteins. ‘na’: not applicable.