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. 2015 Oct 10;43(18):9039–9050. doi: 10.1093/nar/gkv845

Figure 4.

Figure 4.

Thioester conjugation reaction of Rad6 and Rad6L56A mutant monitored by NMR. (A) Formation of the Rad6∼Ub conjugate shows an NMR spectrum that is indicative of oligomerization. TROSY-HSQC NMR spectra are shown as follows; 250 μM 70%-2H,15N-Rad6 (black), 250 μM 70%-2H,15N-Rad6 + 750 μM Ubiquitin (red) and 250 μM 70%-2H, 15N-Rad6, 750 μM Ubiquitin and 10 μM E1 (blue). (B) Oligomerization of mutant Rad6L56A∼Ub conjugates as monitored by TROSY-HSQC NMR spectra of 2H,15N-Rad6L56A and unlabeled ubiquitin as in panel (A).