Figure 2.

Analysis of CRISPR RNA abundance and termini. The coverage plot of S. putrefaciens CN-32 sequence reads (Illumina HiSeq2000) indicates the variable abundance of individual crRNAs. Mature crRNAs contained 5′-terminal 8 nt tags (5′-CUUAGAAA-3′) and 20 nt 3′-ends without further trimming events (inset). Spacers that are marked with an asterisk were tested for interference activity (see Figure 4)