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Characterization of recombinant Cas1821 protein. (A) EMSA assays indicate the binding of 5′-γ[32P]-ATP labeled crRNA by 250 nM Cas1821 (lane 2) or 500 nM Cas1821 (lane 3). Bands were separated by 6% native PAGE. The RNA binding is unspecific and outcompeted by yeast total RNA. (B) Transmission electron micrographs of negatively stained helical structures formed by RNA-bound recombinant Cas1821.
