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. 2015 Oct 10;43(18):8913–8923. doi: 10.1093/nar/gkv882

Figure 6.

Figure 6.

In vivo characterization of Cas1822 point-mutants. (A) S. putrefaciens CN-32 strains carrying Cas1822 proteins with the indicated point mutations were tested for DNA interference using the conjugation assay (see Figure 3). (B) Northern Blot analyses with anti-repeat probes revealed the cellular crRNA pool of these strains.