Table 4. Correlations between experimental (Hsu et al. (2013) (18)) cutting frequencies at target sites containing a single rG·dG, rC·dC, rA·dA and rU·dT mismatch in the PAM-distal region (≥10th protospacer site)^a and measures of guide RNA–protospacer stability.

		log10(P-value)	Correlation coefficient
Hsu et al. (18) estimated cutting frequency versus guide RNA–protospacer binding energyb		−0.4400	(0.0786)
Hsu et al. (18) estimated cutting frequency versus position of mismatch		−5.8258	0.3990
Hsu et al. (18) estimated cutting frequency versus fractional time guide RNA bound at position ≥the mth protospacer site in a simulated R-loop (KMC)c	m = 14	−9.5550	0.5078
	m = 15	−7.4854	0.4522
	m = 16	−6.9510	0.4333
	m = 17	−3.9270	0.3191
	m = 18	−0.7639	(0.1159)
	m = 19	−0.5546	(0.1058)
	m = 20	−0.2346	(−0.0176)

^an = 136.

^bSee Supplementary Methods and Supplementary Table S1 for details.

^cSee text for details. Max(t) = 100.