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. 2015 Oct 10;43(18):9076–9085. doi: 10.1093/nar/gkv901

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Engineered Cre mutants retain preference for heterotetrameric complex in mouse retinal cells. Dissected newborn mouse retinas (with lens in place) were electroporated with constructs encoding: (i) Nrl-eGFP as a control for electroporation efficiency, (ii) a reporter construct for Cre activity comprised of DsRed preceded by a floxed stop codon and (iii) a gene encoding either wild-type (A) or engineered Cre (B–D) under control of the Nrl promoter. The left side of each panel shows the fluorescence from the green channel, which indicates cells that were successfully electroporated. Fluorescence from the red channel results from removal of the floxed stop codon, indicating Cre activity. The lens shows some autofluorescence which is apparent as a central circular region of red fluorescence in B, C and D. (E) Quantification of activity of electroporated constructs relative to wild-type Cre.