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. 2015 Oct 10;43(18):8898–8912. doi: 10.1093/nar/gkv911

Figure 4.

Figure 4.

The STAT3-N domain is responsible for AGG-Luc promoter activation. (A) STAT3, CBP, c-Src and EV expression plasmids were transfected along with corresponding luciferase reporter vector into 293T cells. Luciferase activity was examined 48 h after transfection. Data are representative of three independent experiments, and bar graphs show the mean ± SD with *P < 0.01. (B) Myc-tagged STAT3-full length or indicated STAT3 domains were transfectedinto 293T cells. After 36 h, the cells were suspended in lysis buffer and samples were analyzed by anti-Mycimmunoblotting. (C) Myc-tagged STAT3-FL or indicated STAT3 domains were transfected along with AGG-Luc reporter or SIE-Luc reporter vectors in 293T cells followed by transfection with or without CBP. Luciferase activity was examined 48 h after transfection. Data are representative of three independent experiments, and bar graphs show the mean ± SD, with *P < 0.01.