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. 2015 Sep 8;21(19-20):2559–2571. doi: 10.1089/ten.tea.2015.0105

FIG. 2.

FIG. 2.

Properties of cardiac myocytes derived from hESCs with diminished B2M expression. The top row shows a typical appearance of a differentiated G-Mod cardiomyocyte layer in a phase-contrast (A) and a fluorescence mode (B). The presence of GCaMP sensor in G-Mod cells enables direct monitoring of rhythmical cell contractions upon cardiac lineage differentiation seen as transient increases in endogenous cell fluorescence. The panel (C) shows sarcomeric alpha-actinin immunostaining with regular striations indicative of proper structural development. The panel (D) illustrates changes in relative expression levels of pluripotency (NANOG, Oct3/4), mesodermal (TBX5), early and late (alpha-actin, GATA4, NkX2.5, alpha-2 actinin, myosin heavy-chain 6) markers in samples acquired on day 1, 7, and 21 of cardiac differentiation protocol (n = 3). The panel (E) shows 3D cardiac fiber composed of G-Mod cardiac myocytes with the trace of calcium transients acquired from it to illustrate regular spontaneous contractions.