FIG. 3.

Functional assessment of cardiac myocytes derived from hESCs with diminished B2M expression. (A) There were no detectable functional differences between cardiac myocytes differentiated from G-Par and G-Mod cell lines. Neither the number of days to develop spontaneous contractions nor the average beating frequency or calcium transient time to peak was different between the two cell lines. (B) Typical recordings of calcium transients from spontaneously beating confluent cell monolayers. Frequency of spontaneous beating ranged from 0.1 to 1 Hz for individual coverslips with an average frequency of 0.45 Hz for both cell types. (C) Sparsely plated individual cells contracted less frequently with longer calcium transients, consistent with frequency-dependent acceleration of relaxation frequency.