FIG. 5.

Expression levels of B2M versus the presence of functional human leukocyte antigen (HLA)-I on cell surface. (A) Data show decreased levels of B2M expression in a single round of parallel differentiation of G-Par and G-Mod cells to cardiac lineage. Two different primers (patterned vs. solid bars) mapping two different regions on the B2M gene: the 3′ end B2M-H (589–674) and the two exons spanning B2M-B (322–435) were used in this experiment. (B) Flow cytometry analysis confirmed a significant loss of HLA-I on the cell surface in both undifferentiated and differentiated G-Mod cells, compared to their G-Par counterparts. Thin line corresponds to unstained cell sample.