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. 2015 Aug 25;21(19-20):2504–2514. doi: 10.1089/ten.tea.2014.0607

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Copyright 2015, Mary Ann Liebert, Inc.

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FIG. 3. — Cell adhesion and viability of pulmonary valve endothelial cells (PVECs) and pulmonary valve interstitial cells (PVICs) grown on PC. The upper left hand panel shows morphology of PVECs grown on slides with (A) gelatin, (B) PC without plasma oxidation, (C) PC with plasma oxidation, (D) PC with plasma oxidation and coated with fibronectin, and (E) PC with plasma oxidation coated with collagen. (F) Shows the number of adherent cells on slides treated with 10–1000 μg/mL of either collagen or fibronectin. Coating PC membranes with collagen significantly enhanced PVEC number when compared to cells grown on slides coated with fibronectin (*p<0.001, N=4). The upper right hand panel shows the morphology of PVICs on slides with (G) no coating, (H) PC without plasma oxidation, (I) PC with plasma oxidation and coated with fibronectin, and (J) PC with plasma oxidation coated with collagen. (K) Shows the number of adherent cells on slides treated with 10–1000 μg/mL of either collagen or fibronectin. Coating PC with either collagen enhances PVIC number compared to control and slides coated with fibronectin (^#p<0.05 [control compared to collagen], **p<0.001 [collagen compared to fibronectin], respectively, N=4).