FIG. 4.

Effect of different coatings on the incidence of apoptosis. TUNEL and DAPI staining of PVECs (A–G) and PIVCs (H–N) on different coatings. The negative control is where TdT enzyme was omitted, and the positive control is represented with cells treated with H₂O₂. PVECs and PVICs were grown in standard culture conditions on slides coated with gelatin alone (or plan microscopic slides for PVICs) and slides coated with PC and gelatin (or PC without coating for PVICs), collagen or fibronectin. Cells cultured on PC with different coatings showed very low levels of apoptosis, with no significant difference when compared to cells grown on microscopic slides. *p<0.05. Color images available online at www.liebertpub.com/tea