FIG. 12.
Through-the-thickness vascularization of the layered constructs. (A, C) The representative hematoxylin and eosin-stained low-magnification histological images showing all the layers of nanofibrous–microfibrous as well as nanofibrous ceramic constructs, respectively. (B, D) The representative images showing the presence of functional vessels (arrows) at the interface in middle of the construct. (E) The semiquantification of the number of capillaries present in the core of the construct. *p < 0.05 compared with the number of capillaries in nanofibrous ceramic (NC+CG+C+) and nanofibrous–microfibrous (NC+MG+C+) constructs.