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. Author manuscript; available in PMC: 2015 Oct 14.
Published in final edited form as: Anal Chem. 2007 Dec 7;80(1):48–54. doi: 10.1021/ac701943b

Table 2.

Composition of the Solubilization/Immobilization Buffers Used in the Preparation of CMAC Columnsa

cell type receptor detergent additive
SH-EP1 α7 nAChR 2% cholate CH, PS, PE
SH-EP1 α4β2 nAChR 2% cholate PS, PE
HEK-293 α7 nAChR 2% cholate CH
HEK-293b α4β2 nAChR 2% cholate
HEK-293c β2 AR 2% cholate or CHAPS PC
a

Where nAChR is nicotinic acetyl choline receptor, AR is adrenergic receptor, CH is cholesterol, PS is L-α-phosphatidylserine, PE is L-α-phosphatidylethanolamine, and PC is mixed egg phosphatidylcholine.

b

Data obtained from ref 17.

c

Data obtained from ref 25.