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. Author manuscript; available in PMC: 2015 Oct 14.
Published in final edited form as: Int J Biochem Cell Biol. 2010 Mar 17;42(6):1007–1018. doi: 10.1016/j.biocel.2010.03.002

Fig. 6.

Fig. 6

Chromatographic elution of imipramine from the CMAC-hα4β2 AChR column. (A) Imipramine is eluted from the column with ammonium acetate buffer (10 mM, pH 7.4) and 15% methanol as the mobile phase, at 0.2 mL/min and 20 °C. The dashed line represents the elution of imipramine from the CMAC-hα4β2 AChR column in the presence of κ-BTx (the immobilized AChR is mainly in the resting state), and the straight line represents the elution of imipramine from the CMAC-hα4β2 AChR column in the presence of (±)-epibatidine (the immobilized AChR is mainly in the desensitized state). (B) Imipramine is eluted from the CMAC-hα4β2 AChR column with (±)-epibatidine present in the mobile phase (predominantly desensitized state) at different temperatures (from right to left: 10, 12, 16, 20, and 25 °C).