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. Author manuscript; available in PMC: 2015 Dec 19.
Published in final edited form as: Science. 2015 Jun 19;348(6241):aaa8205. doi: 10.1126/science.aaa8205

Figure 2. Intrauterine immunization with UV-Ct–cSAP protects against challenge with live Ct.

Figure 2

(A) Ct burden following i.u. challenge with live Ct 4 weeks after immunization (n=4-10 mice/group; **P<0.01; ***P<0.001). (B-C) Timecourse of Ct burden following i.u. Ct challenge 4 weeks after immunization (n=4 mice/group/timepoint, ***P<0.001) measured by (B) qPCR or (C) in vitro assessment of inclusion-forming units (IFUs). (D) Ct burden following intravaginal challenge with Cm 4 weeks after immunization with Cm and UV-Cm-cSAP. n = 3-4 mice/group; *** P<0.001. (E) Gross uterine pathology determined as hydrosalpinx score 4 weeks after intravaginal challenge of immunized and naïve mice with Cm (n=8 mice/group; **P<0.01, ***P<0.001. (F) Ct burden following i.u. Ct challenge 6 months after i.u. im munization (n=4-10 mice/group; ***P<0.001). Statistical differences were assessed using one-way ANOVA followed by Bonferroni's post-test.