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. Author manuscript; available in PMC: 2015 Dec 19.
Published in final edited form as: Science. 2015 Jun 19;348(6241):aaa8205. doi: 10.1126/science.aaa8205

Figure 4. UV-Ct-induced tolerance is mediated by FoxP3+ NR-1 cells.

Figure 4

(A-D) Sorted GFP− NR1xFoxP3-eGFP TN were adoptively transferred to naïve mice prior to immunization. Total GFP+CD25+ NR1 cells (CD4+Vβ8.3+Vα2+) were enumerated by FACS in single-cell suspensions of (A, B) iliac LNs and (C, D) uterus 4 days after immunization and are shown as (A, C) representative contour plots of NR1 cells and (B, D) percent of total NR1 cells (n=5 mice/group; **P<0.01). (E) Ct burden following i.u. Ct challenge 4 weeks after immunization with Ct, UV-Ct or UV-Ct–cSAP. In some animals Treg were depleted with anti-CD25 mAb (clone PC61), while the other groups received isotype-matched IgG three days before and after challenge (n=6 mice/group; ***P<0.001). (F) Ct burden following i.u. challenge with live Ct 4 weeks after immunization of Il10−/− mice. (n=5-11 mice/group; *P<0.05; **P<0.01; ***P<0.001). Error bars depict mean ± SEM. Statistical differences were assessed using one-way ANOVA followed by Bonferroni's post-test.