Table 2.
Thickness (in mm) of Different Fibrin BAMs Cultured for 7 Days in EGM-2
| A. Fibrin ECM | B. Fibrin + 10% Matrigel ECM | |
|---|---|---|
| 5 × 105 muscle cells | 3.20 ± 0.07 (n = 3) | 3.23 ± 0.35 (n = 3) |
| 1 × 106 muscle cells | 2.48 ± 0.39 (n = 3) | 3.32 ± 0.13 (n = 3) |
| 5 × 105 muscle cells + 5 × 105 GFP-HUVECs | 2.20 ± 0.36 (n = 3) | 2.20 ± 0.18 (n = 3) |
| 1 × 106 muscle cells + 1 × 106 GFP-HUVECs | 1.27 ± 0.22 (n = 12) | ND |
| 12 × 105 muscle cells + 8 × 105 GFP-HUVECs | 1.30 ± 0.03 (n = 4) | ND |
| 14 × 105 muscle cells + 6 × 105 GFP-HUVECs | 1.28 ± 0.21 (n = 4) | ND |
In the Formation of aligned myofibers and endothelial networks in fibrin BAMs with a coculture of muscle cells and HUVECs section, only column A is discussed. In the Matrigel impacts myofiber distribution, but does not improve myofiber and endothelial network formation section, a comparison is made between column A and B. ND, not determined.