Table 4.
Parameters Characterizing the Formation of Myofibers in Fibrin Coculture BAMs with a Total of 1 × 106 Cells with 50% (A), 40% (B), and 30% (C) HUVECs or with a Total of 2 × 106 Cells with 50% (D), 40% (E), and 30% (F) HUVECs
| A (n = 11) | B (n = 24) | C (n = 19) | D (n = 34) | E (n = 20) | F (n = 15) | A–B | A–C | B–C | D–E | D–F | E–F | A–D | B–E | C–F | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Total # cells in BAM | 1 × 106 | 1 × 106 | 1 × 106 | 2 × 106 | 2 × 106 | 2 × 106 | |||||||||
| # Muscle cells | 5 × 105 | 6 × 105 | 7 × 105 | 10 × 105 | 12 × 105 | 14 × 105 | |||||||||
| # HUVECs | 5 × 105 | 4 × 105 | 3 × 105 | 10 × 105 | 8 × 105 | 6 × 105 | |||||||||
| Number/microscopic field | 25.63 ± 10.02 | 47.08 ± 15.41 | 48.68 ± 12.56 | 40.48 ± 10.25 | 48.29 ± 13.62 | 49.16 ± 7.26 | ** | *** | NS | NS | ** | NS | ** | NS | NS |
| Alignment | 16.24 ± 5.96 | 6.25 ± 0.84 | 7.72 ± 4.30 | 5.09 ± 1.33 | 3.65 ± 0.79 | 4.912 ± 1.12 | *** | * | NS | *** | NS | ** | *** | *** | *** |
| Length (μm) | 118.90 ± 6.93 | 123.4 ± 13.9 | 119.7 ± 15.9 | 127.4 ± 37.56 | 121.3 ± 15.37 | 119.2 ± 13.18 | NS | NS | NS | NS | NS | NS | NS | NS | NS |
| Diameter (μm) | 12.79 ± 4.60 | 16.88 ± 8.82 | 24.05 ± 7.18 | 13.26 ± 3.69 | 12.22 ± 2.88 | 11.96 ± 1.058 | NS | *** | ** | NS | NS | NS | NS | * | *** |
In the last nine columns, statistical significances are shown for comparisons of indicated groups. For multiple comparisons within groups [1 × 106 total cell count (A–C) or 2 × 106 total cell count (D–F)], a nonparametric Kruskal–Wallis test with Dunn's post-test was used. For pairwise comparisons between groups (A–D, B–E, and C–F), a Mann–Whitney test was performed.