Fig 5. Disruption of interaction between ANX2 and p11 suppresses short-term homing and engraftment of primary ALL cells in immune-compromised mice.

A) NSG-B2m mice were injected with either anti-ANX2 antibody or an isotype-matched control antibody (IgG) before transplantation of 10 million NTPL-20 cells. Twenty-four hours post cell injection, mice were sacrificed and peripheral blood, spleen, liver, and femurs were harvested. Femurs were flushed to collect bone marrow. The percentage of human cells was detected by flow cytometry. The error bars denote SE of the Mean (n = 6 each). Asterisks indicate statistical significance with P < 0.05. B) NSG-B2m mice were injected with either anti-p11 antibody or an isotype-matched control antibody (IgG) before transplantation of 10 million NTPL-20 cells. The percentage of human cells in mouse organs was detected by flow cytometry twenty four hours post transplantation as described in A. The error bars denote SE of the Mean (n = 5 each). Asterisks indicate statistical significance with P < 0.05. C) Mice were pre-treated with either ANX2T inhibitor or vehicle before NTPL-20 cell transplantation. The graph shows the percentage of human cells in indicated organs determined twenty-four hours post cell injection. The error bars represent SE of the mean (n = 5). Asterisk denotes P < 0.05. D) Mice were pre-treated with either ANX2T inhibitor or vehicle before NTPL-90 cell transplantation. The graph shows the percentage of human cells in indicated organs determined twenty-four hours post cell injection. The error bars represent SE of the mean (n = 5). Asterisk denotes p < 0.05. E) The graph shows the increase in the percentage of human cells in mouse blood over time. Error bars denote SE of the mean from five mice each. *P < 0.05. F) Percentage of engraftment in bone marrow and spleen in mice at two weeks post transplantation with NTPL-20 cells is shown in the graph. Error bars represent SE of the mean from four mice each. *P < 0.01.