Fig 8. In vivo assessment of the effect of D₂-receptor blockade on the insulin/PI3K/AKT/FOXO1 and mTOR signalling pathway.

Western blotting showing the SULP-mediated AKT phosphorylation that activated FOXO1 in the nucleus, which in turn translocated into the cytoplasm (reduced FOXO1β phosphorylation in the nucleus and increased FOXO1β phosphorylation in the cytoplasm). Numbers in the western blot captures indicate the relative FOXO1β, AKT and mTOR phosphorylation level following treatment compared to the control level that was set at 1. C: controls treated with normal saline; SULP: sulpiride (selective dopamine D₂-antagonist); B[a]P: benzo[a]pyrene; OIL: olive oil.