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. 2015 Oct 14;11(10):e1005562. doi: 10.1371/journal.pgen.1005562

Fig 4. Coat mislocalization in the absence of SpoIIQ and SpoIIIA proteins.

Fig 4

DIC and fluorescence microscopy of WT, spoIIQ −(IIQ –), spoIIIA −(IIIA –), spoIIIAH −(IIIAH –), sigG –, and spoIVA −(IVA –) strains producing CotE-SNAP fusions. Cells were collected after 24 hrs on sporulation media and labeled with the SNAP substrate TMR-Star (green), the lipophilic dye FM4-64 (red), and the Hoechst nucleoid dye (blue). Yellow arrows indicate where the SNAP signal (green) overlaps with FM4-64 staining (red). Scale bars represent 2 μm.