Fig 6. C. difficile SpoIIQ and SpoIIIAH directly interact in vitro.

Western blot analyses of co-affinity purifications of His₆-tagged SpoIIQ with either empty vector (EV), HA-SpoVT, and HA-SpoIIIAH. The indicated constructs were produced in E. coli upon induction with IPTG. Input represents the soluble fraction of cell lysates prepared from the co-expression strains prior to Ni²⁺-affinity purification, while eluate represents the fractions eluted from the Ni²⁺-affinity beads following incubation with the soluble fraction. Dual western blot detection was performed using anti-HA (red) and anti-His (green).