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. 2015 Oct 14;11(10):e1005562. doi: 10.1371/journal.pgen.1005562

Fig 9. Engulfment defective mutants actively remodel peptidoglycan around the forespore.

Fig 9

Strains were induced to sporulate on solid media for 14 hrs then resuspended in liquid sporulation media and incubated with alkyne D-alanine (alkDala). After fixation, permeabilization, and copper-catalyzed cycloaddition of an azide-conjugated fluorophore, Hoechst nucleoid dye was added, and cells were visualized by light microscopy. Scale bars represent 2 μm.