Fig 9. Engulfment defective mutants actively remodel peptidoglycan around the forespore.
Strains were induced to sporulate on solid media for 14 hrs then resuspended in liquid sporulation media and incubated with alkyne D-alanine (alkDala). After fixation, permeabilization, and copper-catalyzed cycloaddition of an azide-conjugated fluorophore, Hoechst nucleoid dye was added, and cells were visualized by light microscopy. Scale bars represent 2 μm.