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. 2015 Oct 14;10(10):e0129244. doi: 10.1371/journal.pone.0129244

Fig 4. Mediation of Src in 4-HNE-induced enhancement of AP-1 activity in YPEN-1 cells.

Fig 4

(A) YPEN-1 cells were transfected with Src siRNA for 48 h, treated with HNE (10 μM), and immunoblotted for c-Jun and phosphor-c-Jun. (B) The nuclear translocation of c-Jun was visualized by immunocytochemical staining. Nuclei were stained with Hoechst dye (blue) and alexa-488-conjugated (green) goat anti-rabbit IgG to detect c-Jun antibody. Merged images are shown on the right. (D) AP-1 activities were measured by luciferase reporter assay in cells transfected with AP-1 luciferase reporter constructs. The Src inhibitor dasatinib (200 nM) was pretreated for 30 min before 4-HNE stimulation. Bars represent means±SEs (n = 4).*p<0.05 vs. control, #p<0.05 vs. vehicle. AP-1, activator protein-1; CON, control; HNE, 4-hydroxy-2-nonenal; NC, negative control; si, Src siRNA treated; TFIIB, transcription factor II B.