Table 2.
Characteristic features of ADAMTS13 assays
| Substrate | Method of detection | Target of detection | Comments | |
|---|---|---|---|---|
| VWF multimers | SDS PAGE and immunoblotting 5 | Proteolytic fragments | Validated in a linkage study 24 Technically demanding |
|
| Multimer analysis and immunoblotting | VWF multimers | Technically demanding | ||
| ELISA-Collagen binding 17 | VWF multimers | Performance is variable 23 | ||
| Ristocetin cofactor activity 14 | VWF multimers | Good performance in a blind study 23 | ||
| IRMA18 | VWF multimers | Limited experience | ||
| ELISA-Ab774619 | VWF multimers | Limited experience | ||
| VWF peptides | GST-1596VWF1668-His | ELISA6 | VWF peptides | Used in our clinical lab |
| Hrp-1591VWF1668-biotin | HRP activity after adsorption with avidin 21 | Proteolytic fragments | Limited experience | |
| His-A2-Tag100 | ELISA20 | VWF peptides | Limited experience | |
| FREST-VWF73 | Fluorescence 16 | Proteolytic fragments | Quick turnaround Cost may be prohibiting |
|