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. 2015 Oct 14;10(10):e0140055. doi: 10.1371/journal.pone.0140055

Fig 2. Follicular proliferation.

Fig 2

Immunohistochemical localization of PCNA in ovarian follicles was studied 4 days after irradiation. (A): Expression of PCNA in the control ovaries shows a large antral follicle (AnF) with positively stained (dark brown) granulosa cells (GC) and oocyte (O) and a cluster of primordial follicles with positively stained oocytes and stain-free pregranulosa cells around (oval). (B): Expression of PCNA in ovaries of rats subjected to γ-irradiation (3.2 Gy) shows minimal expression. (C) & (D): Expression of PCNA in ovaries of rats treated with GH 1 mg/kg for one week [either exposed to γ-radiation (C) or not (D)] shows a high PCNA expression of oocyte (O) and Granulosa cells (GC) (brown color). Scale bar, 20 μm. (E): Quantitative Immunohistochemical staining of PCNA expressed as a percentage of immunopositive cells against the total number of granulosa cells across six high power fields (40×) from each rat section. Bars represent the Mean ± SEM of at least three independent experiments. a or b: Statistically significant from control or radiation group, respectively at P<0.05 using one-way ANOVA followed by Tukey–Kramer as a post-hoc test.