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. 2015 Oct 14;10(10):e0140055. doi: 10.1371/journal.pone.0140055

Fig 6. Immunohistochemical localization and Real-time quantitative Rt-PCR of insulin like growth factor-1 receptor (IGF-1R).

Fig 6

(A): section of ovary obtained from the control rats shows modest degree of IGF-1R expression (brown color). (B): section of ovary obtained from rats subjected to γ- irradiation (3.2 Gy) shows extensive IGF-1R expression (brown color) in granulosa cells of almost all follicles. (C): section of an ovary obtained from rats treated with GH and exposed to γ –irradiation (3.2 Gy) shows high IGF-1R expression (brown color). (D): section of ovary obtained from rats treated with GH alone (1mg/kg; once daily for one week) shows modest IGF-1R expression similar to control ovaries (brown color).Scale bar, 20 μm. (E): Semi-quantitative expression of ovarian IGF-1R staining represents the mean optical density of immunopositive cells of 6 high power fields (20×) of at least three independent experiments. (F): Real-time quantitative RT-PCR of ovarian IGF-1 and IGF-1R genes expressed as fold changes relative to control group. Each bar represents the Mean ± SEM for a group of 3 rats. a or b: Statistically significant from control or radiation group, respectively at P<0.05 using one-way ANOVA followed by Tukey–Kramer as a post-hoc test.