Fig 2. Results from a linearity assessment of a testosterone enzyme-linked immunosorbent assay (ELISA) using green sea turtle plasma extracts (six immature males).
Serial dilutions of a pool of extracted green turtle plasma samples (hollow triangles) show parallelism with serial dilutions of testosterone standards (filled squares). Linearity suggests that the assay is measuring the same antigens in the plasma extracts as in the testosterone standards and, therefore, that the ELISA is valid for use with green sea turtle plasma extracts. % B/B0 = % bound/maximum bound