Fig 3. Akt mediates insulin-induced albumin endocytosis.

Transfection of HKC-8 cells with a double dominant negative Akt construct resulted in a decrease in insulin induced endocytosis (p<0.05). The comparisons were performed between albumin treated and insulin+albumin treated cells. Insulin treatment resulted in a statistically significant increase in albumin uptake. In order to examine the role of Akt in insulin-induced albumin endocytosis, a comparison of albumin uptake was performed between control cells and cells transfected by DDN Akt plasmid after insulin treatment as depicted in the Fig. Western blot images of the samples revealed that cells transfected with DDN had a lower band with exposure to Akt-pser473 antibody (Cell Signaling) which is consistent with the lack of phosphorylation ability at the pser473-residue.