Table 1. Human primer sequences used for RT–PCR.
Gene | Accession number | Primers (5’-3’) | nM |
---|---|---|---|
TGF-β1 |
NM_000660.5 |
F: CTCGCCAGAGTGGTTATCTT |
50 |
R: AGTGTGTTATCCCTGCTGTCA |
|||
TGF-β2 |
NM_003238.3 |
F: GAGGGATCTAGGGTGGAA |
50 |
R: GCTGTGCTGAGTGTCTGAA |
|||
GAPDH |
NM_002046.5 |
F: GAAGGTGAAGGTCGGAGTC |
50 |
R: GAAGATGGTGATGGGATTTC |
|||
ACTIN |
AK225414.1 |
F: CATGTACGTTGCTATCCAGGC |
50 |
R: CTCCTTAATGTCACGCACGAT |
GenBank accession numbers are available at NCBI.