Table 3. Representative quality controls of four cytokines diluted in assay buffer.
Analytes | IL-1β |
IL-6 |
IFN-γ |
TNF-α |
||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Experiment 1 | Experiment 1 | Experiment 1 | Experiment 1 | |||||||||
ID |
expected pg/ml |
Recovery % 2+ |
% CV |
expected pg/ml |
Recovery % 2+ |
% CV |
expected pg/ml |
Recovery % 2+ |
% CV |
expected pg/ml |
Recovery % 2+ |
% CV |
QC1+ |
88–182 |
132 |
6.9 |
96–199 |
100 |
5 |
105–214 |
118 |
3 |
94–208 |
100 |
15 |
QC2+ | 485–1008 | 107 | 3.2 | 532–1105 | 100 | 7 | 566–1176 | 100 | 3 | 483–1003 | 100 | 5.1 |
Experiment 2 | Experiment 2 | Experiment 2 | Experiment 2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
QC1+ |
88–182 |
112 |
2.4 |
96–199 |
100 |
6 |
105–214 |
100 |
0 |
94–208 |
100 |
4.7 |
QC2+ | 485–1008 | 107 | 9.4 | 532–1105 | 100 | 5 | 566–1176 | 101 | 1 | 483–1003 | 100 | 4.3 |
+ QC1, quality control at lower concentration ranges; QC2, quality control at higher concentration ranges. 2+ Measurement that was within the expected ranges was considered as 100% recovery. For the measurement higher than the upper ranges of the expected, the percent recovery is higher than 100%.