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. 2015 Oct 13;21:1151–1161.

Table 3. Representative quality controls of four cytokines diluted in assay buffer.

Analytes IL-1β
IL-6
IFN-γ
TNF-α
Experiment 1 Experiment 1 Experiment 1 Experiment 1
ID
expected pg/ml
Recovery % 2+
% CV
expected pg/ml
Recovery % 2+
% CV
expected pg/ml
Recovery % 2+
% CV
expected pg/ml
Recovery % 2+
% CV
QC1+
88–182
132
6.9
96–199
100
5
105–214
118
3
94–208
100
15
QC2+ 485–1008 107 3.2 532–1105 100 7 566–1176 100 3 483–1003 100 5.1
  Experiment 2 Experiment 2 Experiment 2 Experiment 2
QC1+
88–182
112
2.4
96–199
100
6
105–214
100
0
94–208
100
4.7
QC2+ 485–1008 107 9.4 532–1105 100 5 566–1176 101 1 483–1003 100 4.3

+ QC1, quality control at lower concentration ranges; QC2, quality control at higher concentration ranges. 2+ Measurement that was within the expected ranges was considered as 100% recovery. For the measurement higher than the upper ranges of the expected, the percent recovery is higher than 100%.