Table 1. Primer sequences for selected reference genes.
| Gene name (abbreviation) | Primer sequence 5’– 3’ | Product size (base pairs) | Anneal. temp (°C) | GenBank accession number | Tissues employed | Reference |
|---|---|---|---|---|---|---|
| Hypoxanthine phosphoribosyl-transferase 1 (HPRT1) | F: CACTGGGAAAACAATGCAGA | 123 | 56 | AY_283372 | Liver | [31] |
| R: ACAAAGTCAGGTTTATAGCCAACA | Lymph node | |||||
| Spleen | ||||||
| Hydroxymethyl-bilane synthase (HMBS) | F: TCACCATCGGAGCCATCT | 112 | 57 | XM_546491 | Liver | [31] |
| R: GTTCCCACCACGCTCTTCT | Lymph node | |||||
| Beta 2 –microglobulin (B2M) | F: ACGGAAAGGAGATGAAAGCA | 99 | 56 | XM_535458 | Lymph node | [31] |
| R: CCTGCTCATTGGGAGTGAA | Spleen | |||||
| Succinate dehydrogenase complex subunit A (SDHA) | F: GCCTTGGATCTCTTGATGGA | 92 | 56 | XM_535807 | Liver | [31] |
| R: TTCTTGGCTCTTATGCGATG | Skin | |||||
| Glyceraldehyde– 3—phosphate dehydrogenase (GAPDH) | F: GGAGAAAGCTGCCAAATATG | 193 | 55 | NM_001003142 | Spleen | [32] |
| R: ACCAGGAAATGAGCTTGACA | Skin | |||||
| HIRA interacting protein 5 (HIRP5) | F: GGCTTTGAAGATGGCATTGT | 127 | 56 | XM_850340 | Skin | [33] |
| R: CACCCTCCACCTCTGGAATA |